منابع مشابه
Products from the photolysis of diazoacetyl chymotrypsin.
1. A new and convenient synthesis of p-nitrophenyl diazoacetate has been devised from p-nitrophenyl chloroformate and diazomethane. 2. p-Nitrophenyl diazoacetate reacts stoichiometrically with chymotrypsin to yield diazoacetyl chymotrypsin. 3. Photolysis of the diazoacetyl enzyme with light of wave length greater than 320 rnp yields a mixture of (a) glycolic acid and regenerated chymotrypsin an...
متن کاملPreparation of Isopropyl 2-diazoacetyl(phenyl)carbamate.
HAZARD WARNING TO USERS The procedures on the orgsyn.org website are intended for use only by persons with prior training in the field of organic chemistry. In the checking and editing of these procedures, every effort has been made to identify potentially hazardous steps and to eliminate as much as possible the handling of potentially dangerous materials; safety precautions have been inserted ...
متن کاملOn the reaction of diazoacetyl compounds with pepsin.
Previous studies have shown that diazoacetyl-DL-norleutine methyl ester inactivates pepsin, that Cu(I1) greatly facilitates the reaction, and that in the presence of Cu(I1) substitution occurs specifically at the carboxyl group of an aspartic acid residue in the enzyme. The present experiments were designed to establish the reasons for the speed and specificity of the reaction and the role of t...
متن کاملSubtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships.
Evidence is presented for the complete amino acid sequence of subtilisin Carlsberg. The protein consists of a single peptide chain of 274 residues. Comparison with subtilisin BPN’ shows 84 amino acid differences and 1 additional residue in BPN’. The 84 differences can be accounted for on the basis of single or double nucleotide replacements. Within the subtilisins, there are a number of distinc...
متن کاملSubtilisin modification of monodeamidated ribonuclease-A.
Limited proteolysis of RNAase-Aa(1) (monodeamidated ribonuclease-A) by subtilisin results in the formation of an active RNAase-S type of derivative, namely RNAase-Aa(1)S. RNAase-Aa(1)S was chromatographically distinct from RNAase-S, but exhibited very nearly the same enzymic activity, antigenic conformation and susceptibility to trypsin as did RNAase-S. Fractionation of RNAase-Aa(1)S by trichlo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1973
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.70.4.1132